mouse monoclonal anti rad21  (Santa Cruz Biotechnology)

Journal: EMBO Reports

Article Title: STAG2 loss in Ewing sarcoma alters enhancer-promoter contacts dependent and independent of EWS::FLI1

doi: 10.1038/s44319-024-00303-6

Figure Lengend Snippet: ( A ) Chromatin-bound levels of cohesin subunits and regulators assessed by Chromoflow flow cytometry. A representative experiment comparing A673 WT and STAG2 KO#1 is shown. Similar results were obtained comparing WT or Parental cells and STAG2 KO#1 or KO#2 in two independent experiments. ( B ) Immunoprecipitation of cohesin with anti-SMC1 from the indicated cell extracts followed by immunoblotting of cohesin and regulators. Colored dots below the panels identify each sample. ( C ) Quantification of the amount of the indicated proteins co-immunoprecipitated with anti-SMC1, relative to the amount of RAD21, in these four samples. Gray dots often overlap with black dots. .

Article Snippet: Mouse monoclonal anti-RAD21 clone 53A303; FC: 2 µg/ml , Merck , cat#05-908.

Techniques: Flow Cytometry, Immunoprecipitation, Western Blot

Journal: EMBO Reports

Article Title: STAG2 loss in Ewing sarcoma alters enhancer-promoter contacts dependent and independent of EWS::FLI1

doi: 10.1038/s44319-024-00303-6

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Mouse monoclonal anti-RAD21 clone 53A303; FC: 2 µg/ml , Merck , cat#05-908.

Techniques: Recombinant, Sequencing, Reverse Transcription, SYBR Green Assay, Software, Isolation

Journal: The EMBO Journal

Article Title: Requirement of Nek2a and cyclin A2 for Wapl-dependent removal of cohesin from prophase chromatin

doi: 10.1038/s44318-024-00228-9

Figure Lengend Snippet: ( A ) Chromatin from Nek2aΔMR- and Flag-tagged Pds5b-WT-expressing cells (see column/lane 7 of Fig. ) was benzonase-treated and subjected to Rad21-IP. Precipitated proteins were detected by immunoblotting. Chromatin from Nek2aΔMR- and Flag-tagged Pds5b-3A-expressing cells (see column/lane 9 of Fig. ) served as control. mo mock-IP; KD kinase-dead; transg transgenic; endog endogenous. ( B ) HeLaK cells were transfected with the indicated siRNAs (see Fig. ) and released from G2- into a prometaphase arrest. Chromosomes were isolated and subjected to immunofluorescence microscopy using the indicated antibodies. Arrowheads point at pericentromeric regions as judged by CREST staining. Scale bars = 2.5 μm. .

Article Snippet: Rad21 (B2) Mouse mAb (WB dilution 1:800) , Santa Cruz Biotechnology , Sc-271601.

Techniques: Expressing, Western Blot, Control, Transgenic Assay, Transfection, Isolation, Immunofluorescence, Microscopy, Staining

Journal: The EMBO Journal

Article Title: Requirement of Nek2a and cyclin A2 for Wapl-dependent removal of cohesin from prophase chromatin

doi: 10.1038/s44318-024-00228-9

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Rad21 (B2) Mouse mAb (WB dilution 1:800) , Santa Cruz Biotechnology , Sc-271601.

Techniques: Recombinant, Transduction, Sequencing, Protease Inhibitor, Membrane, Software